ACT001 inhibits the proliferation of non-small cell lung cancer cells by upregulating NKTR expression.

ABSTRACT:

Background

Lung cancer, the primary cause of cancer-related deaths worldwide, is diagnosed at an advanced stage and has a poor prognosis. Non-small cell lung cancer (NSCLC) is a major histological type of lung malignancy. This study investigated the effect of ACT001, a novel sesquiterpene lactone derivative, on the proliferation of NSCLC cells and explored the underlying mechanism.

Methods

The effect of ACT001 on cell proliferation was examined by clone formation and MTT assay. Differentially expressed genes and enrichment pathways were analyzed by RNA-seq. Flow cytometry and cell cycle-related protein expression analysis were performed to study the cell cycle. Phosphorylated AKT was detected to explore the mechanism in natural killer cell triggering receptor (NKTR) KD cells

SUBMITTER: Zhao M 

PROVIDER: S-EPMC9200889 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature