Investigation of Retinal Metabolic Function in Type 1 Diabetic Akita Mice.

ABSTRACT: Diabetic retinopathy (DR) is the leading cause of vision loss in working age adults. Understanding the retinal metabolic response to circulating high glucose levels in diabetic patients is critical for development of new therapeutics to treat DR. Measuring retinal metabolic function using the Seahorse analyzer is a promising technique to investigate the effect of hyperglycemia on retinal glycolysis and mitochondrial respiration. Here, we analyzed the retinal metabolic function in young and old diabetic and control mice. We also compared the expression of key glycolytic enzymes between the two groups. The Seahorse XF analyzer was used to measure the metabolic function of retina explants from young and old type 1 diabetic Akita (Ins2^Akita ) mice and their control littermate

SUBMITTER: Shosha E 

PROVIDER: S-EPMC9201036 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature