The Effect of Normothermic Machine Perfusion on the Immune Profile of Donor Liver.

ABSTRACT:

Background

Normothermic machine perfusion (NMP) allows viability assessment and potential resuscitation of donor livers prior to transplantation. The immunological effect of NMP on liver allografts is undetermined, with potential implications on allograft function, rejection outcomes and overall survival. In this study we define the changes in immune profile of human livers during NMP.

Methods

Six human livers were placed on a NMP device. Tissue and perfusate samples were obtained during cold storage prior to perfusion and at 1, 3, and 6 hours of perfusion. Flow cytometry, immunohistochemistry, and bead-based immunoassays were used to measure leukocyte composition and cytokines in the perfusate and within the liver tissue. Mean values between baseline and time points were co

SUBMITTER: Lee ACH 

PROVIDER: S-EPMC9201055 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature