Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis.

ABSTRACT: Here, we describe a protocol for extraction and tandem immunoprecipitation of the cytoplasmic and nuclear proteins from mouse testis for mass spectrometry. This protocol has been applied to knockin mice that express a meiotic protein of interest tagged with 3xFLAG-HA in the testis. The protocol is optimized for salt extraction of cytoplasmic and nuclear proteins from mouse frozen testes and thus can be used for a variety of proteins. For complete details on the use and execution of this protocol, please refer to Ishiguro et al. (2020) and Tanno et al. (2022).

SUBMITTER: Tani N 

PROVIDER: S-EPMC9201064 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature