The silencing of miR-199a-5p protects the articular cartilage through MAPK4 in osteoarthritis.

ABSTRACT:

Background

Osteoarthritis (OA) is the most common joint disorder, and places a heavy burden on individuals and society. As conventional therapies, such as surgery, rarely cure the disorder, targeted therapies represent a promising alternative. This research sought to explore the potential effect of miR-199a-5p on the development of OA.

Methods

Based on the OA rat model, the serum was collected at 6 and 12 weeks, and microRNA (miRNA) sequencing was performed. A bioinformatics analysis was conducted to examine the differentially expressed micro ribonucleic acids, and qRT-PCR (real-time quantitative PCR) was conducted to determine their expression in the joint tissues of rats with OA. Rats articular chondrocytes were collected and treated with a miR-199a-5p antagomir or agomir.

SUBMITTER: Lu H 

PROVIDER: S-EPMC9201181 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature