Hoxb5 reprogrammes murine multipotent blood progenitors into haematopoietic stem cell-like cells.

ABSTRACT:

Objectives

The expression of transcription factor Hoxb5 specifically marks the functional haematopoietic stem cells (HSC) in mice. However, our recent work demonstrated that ectopic expression of Hoxb5 exerted little effect on HSC but could convert B-cell progenitors into functional T cells in vivo. Thus, cell type- and development stage-specific roles of Hoxb5 in haematopoietic hierarchy await more extensive exploration. In this study, we aim to investigate the effect of Hoxb5 expression in multipotent blood progenitor cells.

Materials and methods

A Mx1cre/Rosa^{LSL-Hoxb5-EGFP/+} mouse model was used to evaluate the effect of Hoxb5 expression in blood multipotent progenitor cells (MPP). Golden standard serial transplantation experiments were used to test the long-te

SUBMITTER: Huang D 

PROVIDER: S-EPMC9201374 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature