Exploiting salivary miR-375 as a clinical biomarker of oral potentially malignant disorder.

ABSTRACT:

Background/purpose

Oral potentially malignant disorder (OPMD) is an important premalignancy worldwide. MicroRNAs (miRNAs) are endogenously expressed non-coding RNAs that regulate the post-transcriptional levels of targeted mRNAs. MiRNA-375 (miR-375) is markedly downregulated in oral carcinoma tissues and plays an oncogenic role in oral carcinogenesis. We explored the potential of the deregulated salivary miR-375 levels in OPMD patients.

Materials and methods

. We analyzed the levels of miR-375 in the saliva of patients with OPMD (n = 45) and healthy controls (n = 24) by quantitative RT-PCR. The cell lysates and supernatants were treated with the miR-375 mimic and inhibitor.

Results

Salivary miR-375 levels were de

SUBMITTER: Tu HF 

PROVIDER: S-EPMC9201538 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature