Modulated nanowire scaffold for highly efficient differentiation of mesenchymal stem cells.

ABSTRACT:

Background

Nanotopographical cues play a critical role as drivers of mesenchymal stem cell differentiation. Nanowire scaffolds, in this regard, provide unique and adaptable nanostructured surfaces with focal points for adhesion and with elastic properties determined by nanowire stiffness.

Results

We show that a scaffold of nanowires, which are remotely actuated by a magnetic field, mechanically stimulates mesenchymal stem cells. Osteopontin, a marker of osteogenesis onset, was expressed after cells were cultured for 1 week on top of the scaffold. Applying a magnetic field significantly boosted differentiation due to mechanical stimulation of the cells by the active deflection of the nanowire tips. The onset of differentiation was reduced to 2 days of culture based on the upr

SUBMITTER: Perez JE 

PROVIDER: S-EPMC9202102 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature