MiR-188-3p-targeted regulation of ATG7 affects cell autophagy in patients with nonobstructive azoospermia.

ABSTRACT:

Background

Nonobstructive azoospermia (NOA) is one of the most difficult forms of male infertility to treat, and its pathogenesis is still unclear. miRNAs can regulate autophagy by affecting their target gene expression. Our previous study found that miR-188-3p expression in NOA patients was low. There are potential binding sites between the autophagy gene ATG7 and miR-188-3p. This study aimed to verify the binding site between miR-188-3p and ATG7 and whether miR-188-3p affects autophagy and participates in NOA by regulating ATG7 to influence the autophagy marker genes LC3 and Beclin-1.

Methods

Testicular tissue from 16 NOA patients and 16 patients with normal spermatogenesis and 5 cases in each group of pathological sections were collected. High-throughput sequencing was pe

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9202134 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature