Calcium-dependent cytosolic phospholipase A₂ activation is implicated in neuroinflammation and oxidative stress associated with ApoE4.

ABSTRACT:

Background

Apolipoprotein E4 (APOE4) is associated with a greater response to neuroinflammation and the risk of developing late-onset Alzheimer's disease (AD), but the mechanisms for this association are not clear. The activation of calcium-dependent cytosolic phospholipase A₂ (cPLA2) is involved in inflammatory signaling and is elevated within the plaques of AD brains. The relation between APOE4 genotype and cPLA2 activity is not known.

Methods

Mouse primary astrocytes, mouse and human brain samples differing by APOE genotypes were collected for measuring cPLA2 expression, phosphorylation, and activity in relation to measures of inflammation and oxidative stress.

Results

Greater cPLA2 phosphorylation, cPLA2 activity and leukotriene B4 (LTB4) levels were id

SUBMITTER: Wang S 

PROVIDER: S-EPMC9202185 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature