Hematopoietic differentiation persists in human iPSCs defective in de novo DNA methylation.

ABSTRACT:

Background

DNA methylation is involved in the epigenetic regulation of gene expression during developmental processes and is primarily established by the DNA methyltransferase 3A (DNMT3A) and 3B (DNMT3B). DNMT3A is one of the most frequently mutated genes in clonal hematopoiesis and leukemia, indicating that it plays a crucial role for hematopoietic differentiation. However, the functional relevance of Dnmt3a for hematopoietic differentiation and hematological malignancies has mostly been analyzed in mice, with the specific role for human hematopoiesis remaining elusive. In this study, we therefore investigated if DNMT3A is essential for hematopoietic differentiation of human induced pluripotent stem cells (iPSCs).

Results

We generated iPSC lines with knockout of either exon

SUBMITTER: Cypris O 

PROVIDER: S-EPMC9202186 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature