Combined analysis of T cell activation and T cell-mediated cytotoxicity by imaging cytometry.

ABSTRACT: Immunotherapies for the treatment of cancer have spurred the development of new drugs that seek to harness the ability of T cells to recognize and kill malignant cells. There is a substantial need to evaluate how these experimental drugs influence T cell functional outputs in co-culture systems that contain cancerous cells. We describe an imaging cytometry-based platform that can simultaneously quantify activated T cells and the capacity of these T cells to kill cancer cells. Our platform was developed using the Nur77-GFP reporter system because GFP expression provides a direct readout of T cell activation that is induced by T cell antigen receptor (TCR) signaling. We combined the Nur77-GFP reporter system with a cancer cell line that displays a TCR-specific antigen and evaluated the relat

SUBMITTER: Chanda MK 

PROVIDER: S-EPMC9202232 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature