TALE.Sense: A Versatile DNA Sensor Platform for Live Mammalian Cells.

ABSTRACT: Here we describe TALE.Sense, a versatile platform for sensing DNA sequences in live mammalian cells enabling programmable generation of a customable response that discerns cells containing specified sequence targets. The platform is based on the programmable DNA binding of transcription activator-like effector (TALE) coupled to conditional intein-reconstitution producing a trans-spliced ON-switch for a response circuit. TALE.Sense shows higher efficiency and dynamic range when compared to the reported zinc-finger based DNA-sensor in detecting same DNA sequences. Swapping transcriptional activation modules and introducing SunTag-based amplification loops to TALE.Sense circuits augment detection efficiency of the DNA sensor. The TALE.Sense platform shows versatility when applied to a range o

SUBMITTER: Taghbalout A 

PROVIDER: S-EPMC9202581 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature