Ontology highlight
ABSTRACT:
SUBMITTER: Clark NE
PROVIDER: S-EPMC9202583 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Clark Nathaniel E NE Katolik Adam A Taggart Allison J AJ Buerer Luke L Holloway Stephen P SP Miller Nathaniel N Phillips John D JD Farrell Colin P CP Damha Masad J MJ Fairbrother William G WG
RNA (New York, N.Y.) 20220422 7
In eukaryotic cells, intron lariats produced by the spliceosome contain a 2'5' phosphodiester linkage. The RNA lariat debranching enzyme, Dbr1, is the only enzyme known to hydrolyze this bond. Dbr1 is a member of the metallophosphoesterase (MPE) family of enzymes, and recent X-ray crystal structures and biochemistry data demonstrate that Dbr1 from <i>Entamoeba histolytica</i> uses combinations of Mn<sup>2+</sup>, Zn<sup>2+</sup>, and Fe<sup>2+</sup> as enzymatic cofactors. Here, we examine the k ...[more]