Creeping yeast: a simple, cheap and robust protocol for the identification of mating type in Saccharomyces cerevisiae.

ABSTRACT: Saccharomyces cerevisiae is an exceptional genetic system, with genetic crosses facilitated by its ability to be maintained in haploid and diploid forms. Such crosses are straightforward if the mating type/ploidy of the strains is known. Several techniques can determine mating type (or ploidy), but all have limitations. Here, we validate a simple, cheap and robust method to identify S. cerevisiae mating types. When cells of opposite mating type are mixed in liquid media, they 'creep' up the culture vessel sides, a phenotype that can be easily detected visually. In contrast, mixtures of the same mating type or with a diploid simply settle out. The phenotype is observable for several days under a range of routine growth conditions and with different media/strains. Microscopy suggests that ce

SUBMITTER: Arras SDM 

PROVIDER: S-EPMC9202641 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature