Identification and Isolation of Type II NKT Cell Subsets in Human Blood and Liver.

ABSTRACT:

Background

Steatotic livers are more prone to rejection, but are often transplanted owing to the shortage of available organs. Type II NKT (T2NKT) cells are liver-resident lymphocytes that react to lipids presented by CD1d. The role of T2NKT cells in rejection of fatty liver transplants is unclear, partly because of a lack of T2NKT cell markers and their very low frequency in blood. Here, we quantify human T2NKT cells in blood and liver tissue by flow cytometry and provide a strategy for their enrichment and expansion.

Methods

Human T2NKT cells were identified as CD3⁺ CD56⁺ CD161⁺ TCR-γᵹ^- TCRVα7.2^- and TCRVα24^- cells. T2NKT cells were enriched from blood by sequential positive selection using CD56 and CD3 micr

SUBMITTER: Yang Zhou J 

PROVIDER: S-EPMC9202826 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature