AlphaFold2 and RoseTTAFold predict posttranslational modifications. Chromophore formation in GFP-like proteins.

ABSTRACT: AlphaFold2 and RoseTTAfold are able to predict, based solely on their sequence whether GFP-like proteins will post-translationally form a chromophore (the part of the protein responsible for fluorescence) or not. Their training has not only taught them protein structure and folding, but also chemistry. The structures of 21 sequences of GFP-like fluorescent proteins that will post-translationally form a chromophore and of 23 GFP-like non-fluorescent proteins that do not have the residues required to form a chromophore were determined by AlphaFold2 and RoseTTAfold. The resultant structures were mined for a series of geometric measurements that are crucial to chromophore formation. Statistical analysis of these measurements showed that both programs conclusively distinguished between chromoph

SUBMITTER: Hartley SM 

PROVIDER: S-EPMC9202861 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature