Functional dissection of neural circuitry using a genetic reporter for fMRI.

ABSTRACT: The complex connectivity of the mammalian brain underlies its function, but understanding how interconnected brain regions interact in neural processing remains a formidable challenge. Here we address this problem by introducing a genetic probe that permits selective functional imaging of distributed neural populations defined by viral labeling techniques. The probe is an engineered enzyme that transduces cytosolic calcium dynamics of probe-expressing cells into localized hemodynamic responses that can be specifically visualized by functional magnetic resonance imaging. Using a viral vector that undergoes retrograde transport, we apply the probe to characterize a brain-wide network of presynaptic inputs to the striatum activated in a deep brain stimulation paradigm in rats. The results rev

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC9203076 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature