Correlative imaging of the spatio-angular dynamics of biological systems with multimodal instant polarization microscope.

ABSTRACT: The spatial and angular organization of biological macromolecules is a key determinant, as well as informative readout, of their function. Correlative imaging of the dynamic spatio-angular architecture of cells and organelles is valuable, but remains challenging with current methods. Correlative imaging of spatio-angular dynamics requires fast polarization-, depth-, and wavelength-diverse measurement of intrinsic optical properties and fluorescent labels. We report a multimodal instant polarization microscope (miPolScope) that combines a broadband polarization-resolved detector, automation, and reconstruction algorithms to enable label-free imaging of phase, retardance, and orientation, multiplexed with fluorescence imaging of concentration, anisotropy, and orientation of molecules at diff

SUBMITTER: Ivanov IE 

PROVIDER: S-EPMC9203109 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature