Proton-gated anion transport governs macropinosome shrinkage.

ABSTRACT: Intracellular organelles change their size during trafficking and maturation. This requires the transport of ions and water across their membranes. Macropinocytosis, a ubiquitous form of endocytosis of particular importance for immune and cancer cells, generates large vacuoles that can be followed optically. Shrinkage of macrophage macropinosomes depends on TPC-mediated Na⁺ efflux and Cl^- exit through unknown channels. Relieving osmotic pressure facilitates vesicle budding, positioning osmotic shrinkage upstream of vesicular sorting and trafficking. Here we identify the missing macrophage Cl^- channel as the proton-activated Cl^- channel ASOR/TMEM206. ASOR activation requires Na⁺-mediated depolarization and luminal acidification by redun

SUBMITTER: Zeziulia M 

PROVIDER: S-EPMC9203271 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature