Integrated multi-omics reveal polycomb repressive complex 2 restricts human trophoblast induction.

ABSTRACT: Human naive pluripotent stem cells have unrestricted lineage potential. Underpinning this property, naive cells are thought to lack chromatin-based lineage barriers. However, this assumption has not been tested. Here we define the chromatin-associated proteome, histone post-translational modifications and transcriptome of human naive and primed pluripotent stem cells. Our integrated analysis reveals differences in the relative abundance and activities of distinct chromatin modules. We identify a strong enrichment of polycomb repressive complex 2 (PRC2)-associated H3K27me3 in the chromatin of naive pluripotent stem cells and H3K27me3 enrichment at promoters of lineage-determining genes, including trophoblast regulators. PRC2 activity acts as a chromatin barrier restricting the differentiati

SUBMITTER: Zijlmans DW 

PROVIDER: S-EPMC9203278 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature