Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation.

ABSTRACT: Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (Scherer et al. in J Exp Med 97:695-710, 1953). They not only allow detailed investigation of molecular pathways but are faster and more cost-effective than most in vivo approaches. The last decade saw many emerging model systems strengthening basic science research. However, lack of genetic and molecular tools in these newer systems pose many obstacles. Astyanax mexicanus is proving to be an interesting new model system for understanding metabolic adaptation. To further enhance the utility of this system, we developed liver-derived cell lines from both surface-dwelling and cave-dwelling morphotypes. In this study, we provide detailed methodology of t

SUBMITTER: Krishnan J 

PROVIDER: S-EPMC9203785 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature