Simple and automatic monitoring of cancer cell invasion into an epithelial monolayer using label-free holographic microscopy.

ABSTRACT: The invasiveness of cancer cells describes the metastasizing capability of a primary tumor. The straightforward detection and quantification of cancer cell invasion are important to predict the survival rate of a cancer patient and to test how anti-cancer compounds influence cancer progression. Digital holographic microscopy based M4 Holomonitor (HM) is a technique that allows the label-free monitoring of cell morphological and kinetical parameters in real-time. Here, a fully confluent epithelial monolayer derived from the African green monkey kidney (Vero) on a gelatin-coated surface was established, then HeLa cells were seeded on top of the monolayer, and their behavior was monitored for 24 h using HM. Several cancer cells showing invasiveness were detected during this period, while othe

SUBMITTER: Nagy AG 

PROVIDER: S-EPMC9203807 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature