Tangential flow filtration facilitated washing of human red blood cells: A proof-of-concept study.

ABSTRACT:

Background and objectives

Red blood cell (RBC) units in hypothermic storage degrade over time, commonly known as the RBC storage lesion. These older RBC units can cause adverse clinical effects when transfused, as older RBCs in the unit lyse and release cell-free haemoglobin (Hb), a potent vasodilator that can elicit vasoconstriction, systemic hypertension and oxidative tissue injury after transfusion. In this study, we examined a novel method of washing ex vivo stored single RBC units to remove accumulated cellular waste, specifically cell-free Hb, using tangential flow filtration (TFF) driven by a centrifugal pump.

Materials and methods

The TFF RBC washing system was run under hypothermic conditions at 4°C, at a constant system volume with 0.9 wt% saline as the wash soluti

SUBMITTER: Lu S 

PROVIDER: S-EPMC9203871 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature