A general protocol for the expression and purification of the intact transmembrane transporter FeoB.

ABSTRACT: Ferrous iron (Fe²⁺) transport is an essential process that supports the growth, intracellular survival, and virulence of several drug-resistant pathogens, and the ferrous iron transport (Feo) system is the most important and widespread protein complex that mediates Fe²⁺ transport in these organisms. The Feo system canonically comprises three proteins (FeoA/B/C). FeoA and FeoC are both small, accessory proteins localized to the cytoplasm, and their roles in the Fe²⁺ transport process have been of great debate. FeoB is the only wholly-conserved component of the Feo system and serves as the inner membrane-embedded Fe²⁺ transporter with a soluble G-protein-like N-terminal domain. In vivo studies have underscored the importance of Feo during infection

SUBMITTER: Sestok AE 

PROVIDER: S-EPMC9203943 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature