Epigenetic Clocks for Mice Based on Age-Associated Regions That are Conserved Between Mouse Strains and Human.

ABSTRACT: Aging of mice can be tracked by DNA methylation changes at specific sites in the genome. In this study, we used the recently released Infinium Mouse Methylation BeadChip to compare such epigenetic modifications in C57BL/6 (B6) and DBA/2J (DBA) mice. We observed marked differences in age-associated DNA methylation in these commonly used inbred mouse strains, indicating that epigenetic clocks for one strain cannot be simply applied to other strains without further verification. In B6 mice age-associated hypomethylation prevailed with focused hypermethylation at CpG islands, whereas in DBA mice CpG islands revealed rather hypomethylation upon aging. Interestingly, the CpGs with highest age-correlation were still overlapping in B6 and DBA mice and included the genes Hsf4, Prima1,

SUBMITTER: Perez-Correa JF 

PROVIDER: S-EPMC9204067 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature