Evaluation of an Antibiotic Cocktail for Fecal Microbiota Transplantation in Mouse.

ABSTRACT:

Objective

This study aimed to evaluate the effect of an antibiotic cocktail on gut microbiota and provide a reference for establishing an available mouse model for fecal microbiota transplantation (FMT) of specific microbes.

Design

C57BL/6J mice (n = 24) had free access to an antibiotic cocktail containing vancomycin (0.5 g/L), ampicillin (1 g/L), neomycin (1 g/L), and metronidazole (1 g/L) in drinking water for 3 weeks. Fecal microbiota was characterized by 16S rDNA gene sequencing at the beginning, 1st week, and 3rd week, respectively. The mice were then treated with fecal microbiota from normal mice for 1 week to verify the efficiency of FMT.

Results

The diversity of microbiota including chao1, observed species, phylogenetic diversity (PD) whole tree, and Sh

SUBMITTER: Tan J 

PROVIDER: S-EPMC9204140 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature