Stimulatory G-Protein α Subunit Modulates Endothelial Cell Permeability Through Regulation of Plasmalemma Vesicle-Associated Protein.

ABSTRACT: Endothelial cell leakage occurs in several diseases. Intracellular junctions and transcellular fashion are involved. The definite regulatory mechanism is complicated and not fully elucidated. The alpha subunit of the heterotrimeric G-stimulatory protein (Gsα) mediates receptor-stimulated production of cyclic adenosine monophosphate (cAMP). However, the role of Gsα in the endothelial barrier remains unclear. In this study, mice with knockout of endothelial-specific Gsα (Gsα^ECKO) were generated by crossbreeding Gsα^flox/flox mice with Cdh5-CreER^T2 transgenic mice, induced in adult mice by tamoxifen treatment. Gsα^ECKO mice displayed phenotypes of edema, anemia, hypoproteinemia and hyperlipoproteinemia, which indicates impaired microvascular permeabil

SUBMITTER: He L 

PROVIDER: S-EPMC9204201 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature