Distinct effects of volatile and intravenous anaesthetics on presynaptic calcium dynamics in mouse hippocampal GABAergic neurones.

ABSTRACT:

Background

General anaesthetics have marked effects on synaptic transmission, but their neuronal and circuit-level effects remain unclear. The volatile anaesthetic isoflurane differentially inhibits synaptic vesicle exocytosis in specific neuronal subtypes, but whether other common anaesthetics also have neurone-subtype-specific actions is unknown.

Methods

We used the genetically encoded fluorescent Ca²⁺ sensor GCaMP6f to compare the pharmacological effects of isoflurane, sevoflurane, propofol, and ketamine on presynaptic excitability in hippocampal glutamatergic neurones and in hippocampal parvalbumin-, somatostatin-, and vasoactive intestinal peptide-expressing (PV⁺, SST⁺, and VIP⁺, respectively) GABAergic interneurones.

Resul

SUBMITTER: Speigel IA 

PROVIDER: S-EPMC9204660 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature