Molecular characterization of varicella zoster virus isolated from clinical samples in India.

ABSTRACT:

Background & objectives

Varicella zoster virus (VZV) strains are classified into six different clades based on the sequencing of its genome. Clades 4 and 5 are reported from India based on the single-nucleotide polymorphism (SNP). Till now, multiple clade circulations using partial sequences have been reported from India due to the lack of availability of the full VZV genome sequence. This study conducted a genome sequencing of VZV in India to identify circulating clade.

Methods

Four clinical samples obtained from symptomatic patients tested positive for VZV by real-time PCR were used. These four samples were preferred to retrieve the genomic VZV sequence using the next-generation sequencing method. A reference-based assembly method was used to retrieve the genome of VZV, wh

SUBMITTER: Nyayanit DA 

PROVIDER: S-EPMC9205007 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature