Stimulated phosphorylation of ERK in mouse kidney mesangial cells is dependent upon expression of Cav3.1.

ABSTRACT:

Background

T-type calcium channels (TTCC) are low voltage activated channels that are widely expressed in the heart, smooth muscle and neurons. They are known to impact on cell cycle progression in cancer and smooth muscle cells and more recently, have been implicated in rat and human mesangial cell proliferation. The aim of this study was to investigate the roles of the different isoforms of TTCC in mouse mesangial cells to establish which may be the best therapeutic target for treating mesangioproliferative kidney diseases.  METHODS: In this study, we generated single and double knockout (SKO and DKO) clones of the TTCC isoforms Ca_V3.1 and Ca_V3.2 in mouse mesangial cells using CRISPR-cas9 gene editing. The downstream signals linked to this channel activity w

SUBMITTER: Pandi SPS 

PROVIDER: S-EPMC9205043 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature