Acidification does not alter the stable isotope composition of bone collagen.

ABSTRACT: In this study, we compared the elemental and isotopic composition of modern and ancient bone samples pre-treated using different demineralization agents with acidic and neutral pH. The purpose of our research was to examine if demineralization using a mineral acid such as hydrochloric acid (HCl) significantly alters the δ ¹⁵N and δ ¹³C values of bone collagen. Evidence from the elemental and amino acid composition of the samples were incorporated alongside isotopic compositions to provide a holistic view of the effect of demineralization agents on the composition of bone collagen. The stable isotope compositions of collagen extracts were also compared against equivalent whole bone samples to assess whether whole bone has a stable isotope composition that

SUBMITTER: Wilson T 

PROVIDER: S-EPMC9205305 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature