Whole-genome circulating tumor DNA methylation landscape reveals sensitive biomarkers of breast cancer.

ABSTRACT: The changes in circulating tumor DNA (ctDNA) methylation are believed to be early events in breast cancer initiation, which makes them suitable as promising biomarkers for early diagnosis. However, applying ctDNA in breast cancer early diagnosis remains highly challenging due to the contamination of background DNA from blood and low DNA methylation signals. Here, we report an improved way to extract ctDNA, reduce background contamination, and build a whole-genome bisulfite sequencing (WGBS) library from different stages of breast cancer. We first compared the DNA methylation data of 74 breast cancer patients with those of seven normal controls to screen candidate methylation CpG site biomarkers for breast cancer diagnosis. The obtained 26 candidate ctDNA methylation biomarkers produced hig

SUBMITTER: Hai L 

PROVIDER: S-EPMC9205580 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature