Sequential compound fusion and kiss-and-run mediate exo- and endocytosis in excitable cells.

ABSTRACT: Vesicle fusion at preestablished plasma membrane release sites releases transmitters and hormones to mediate fundamental functions like neuronal network activities and fight-or-flight responses. This half-a-century-old concept-fusion at well-established release sites in excitable cells-needs to be modified to include the sequential compound fusion reported here-vesicle fusion at previously fused Ω-shaped vesicular membrane. With superresolution STED microscopy in excitable neuroendocrine chromaffin cells, we real-time visualized sequential compound fusion pore openings and content releases in generating multivesicular and asynchronous release from single release sites, which enhances exocytosis strength and dynamic ranges in excitable cells. We also visualized subsequent compound fusion po

SUBMITTER: Ge L 

PROVIDER: S-EPMC9205584 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature