An optimization method for untargeted MS-based isotopic tracing investigations of metabolism

ABSTRACT:

Introduction

Stable isotope tracer studies are increasingly applied to explore metabolism from the detailed analysis of tracer incorporation into metabolites. Untargeted LC/MS approaches have recently emerged and provide potent methods for expanding the dimension and complexity of the metabolic networks that can be investigated. A number of software tools have been developed to process the highly complex MS data collected in such studies; however, a method to optimize the extraction of valuable isotopic data is lacking.

Objectives

To develop and validate a method to optimize automated data processing for untargeted MS-based isotopic tracing investigations of metabolism.

Methods

The method is based on the application of a suitable reference material to rationally

SUBMITTER: Butin N 

PROVIDER: S-EPMC9205802 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature