3D structure and in situ arrangements of CatSper channel in the sperm flagellum.

ABSTRACT: The sperm calcium channel CatSper plays a central role in successful fertilization as a primary Ca²⁺ gateway. Here, we applied cryo-electron tomography to visualize the higher-order organization of the native CatSper complex in intact mammalian sperm. The repeating CatSper units form long zigzag-rows along mouse and human sperm flagella. Above each tetrameric channel pore, most of the extracellular domains form a canopy that interconnects to a zigzag-shaped roof. Murine CatSper contains an additional wing-structure connected to the tetrameric channel. The intracellular domains link two neighboring channels to a diagonal array, suggesting a dimer formation. Fitting of an atomic model of isolated monomeric CatSper to the in situ map reveals supramolecular interactions and assembly

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC9205950 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature