Imaging peripheral nerve micro-anatomy with MUSE, 2D and 3D approaches.

ABSTRACT: Understanding peripheral nerve micro-anatomy can assist in the development of safe and effective neuromodulation devices. However, current approaches for imaging nerve morphology at the fiber level are either cumbersome, require substantial instrumentation, have a limited volume of view, or are limited in resolution/contrast. We present alternative methods based on MUSE (Microscopy with Ultraviolet Surface Excitation) imaging to investigate peripheral nerve morphology, both in 2D and 3D. For 2D imaging, fixed samples are imaged on a conventional MUSE system either label free (via auto-fluorescence) or after staining with fluorescent dyes. This method provides a simple and rapid technique to visualize myelinated nerve fibers at specific locations along the length of the nerve and perform me

SUBMITTER: Kolluru C 

PROVIDER: S-EPMC9205958 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature