Identification of storage conditions stabilizing extracellular vesicles preparations.

ABSTRACT: Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological processes and hold great potential for therapeutic and diagnostic use. Despite significant advances within the last decade, the key issue of EV storage stability remains unresolved and under investigated. Here, we aimed to identify storage conditions stabilizing EVs and comprehensively compared the impact of various storage buffer formulations at different temperatures on EVs derived from different cellular sources for up to 2 years. EV features including concentration, diameter, surface protein profile and nucleic acid contents were assessed by complementary methods, and engineered EVs containing fluorophores or functionalized surface proteins were utilized to compare cellular uptake and ligand bindi

SUBMITTER: Gorgens A 

PROVIDER: S-EPMC9206228 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature