Isolation, discrimination, and molecular detection of Listeria species from slaughtered cattle in Namwala District, Zambia.

ABSTRACT:

Background

The food industry is increasingly becoming more scrutinized, given the frequency and intensity with which zoonotic diseases are being reported. Pathogen tracking has become more applicable with regards food safety. It is in this regard that the present study was formulated to track Listeria species. in freshly slaughtered cattle carcasses by utilizing standard and molecular biological techniques.

Methods

A cross-sectional study design was conducted from March to December 2020 with 200 samples being equally collected in the rainy and dry seasons. A total of 180 and 20 swabs were aseptically collected from carcasses and the environment respectively. Samples were first subjected to pre-enrichment in half-strength Fraser broth followed by enrichment in full strength F

SUBMITTER: Mpundu P 

PROVIDER: S-EPMC9206240 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature