Determine both the conformation and orientation of a specific residue in α-synuclein(61-95) even in monolayer by ¹³C isotopic label and p-polarized multiple-angle incidence resolution spectrometry (pMAIRS).

ABSTRACT: Protein's magic function stems from its structure and various analytical techniques have been developed for it. Among proteins, membrane proteins are encoded 20-30% of genomes, whereas cause challenges for many analytical techniques. For example, lots of membrane proteins cannot form single crystal structure required by X-ray crystallography. As for NMR, the measurements were hindered by the low tumbling rates of membrane (i.e., phospholipid bilayers) where membrane proteins exist. In addition, membrane proteins usually lay parallel to the surface of phospholipid bilayers or form transmembrane structure. No matter parallel or perpendicular to phospholipid bilayers surface, membrane proteins form monolayer structure which is also difficult for X-ray and NMR to provide high-resolution result

SUBMITTER: Wang C 

PROVIDER: S-EPMC9206922 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature