Antifungal effect of triclosan on Aspergillus fumigatus: quorum quenching role as a single agent and synergy with liposomal amphotericin-B.

ABSTRACT: The purpose of this research was to determine Aspergillus fumigatus conidial viability and its biofilm formation upon treatment with triclosan and amphotericin-B loaded liposomes. A. fumigatus was treated with the antimicrobials, triclosan and liposomal amphotericin-B (L-AMB), in single and combined supplementation. To quantify the cells' viability upon treatments, resazurin-based viability assay was performed. Confocal laser scanning microscopy was done by applying FUN-1 stain to screen the role of the agents on extracellular polymeric substances. Total A. fumigatus biomass upon treatments was estimated by using crystal violet-based assay. To study the agents' effect on the conidial viability, flow cytometry analysis was performed. Expression levels of A. fumigatus genes encoding cell wal

SUBMITTER: Tamimi R 

PROVIDER: S-EPMC9206924 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature