Development of a novel feeding regime for large scale production of human umbilical cord mesenchymal stem/stromal cells.

ABSTRACT: Human umbilical cord mesenchymal stem/stromal cells (hUC-MSCs) have attracted significant research interests in regenerative medicine and cell-based therapies due to their minimally invasive isolation procedure, abundant availability, allogenic nature, improved proliferation capacity and tri-lineage differentiation potential. However, the challenge in harvesting a sufficient number of hUC-MSCs through conventional static culture for downstream application hinders the downstream clinical translation of hUC-MSCs. Hence, an alternative culture method that can facilitate large-scale expansion is highly desirable. Herein, we developed a microcarrier-based dynamic culture system to culture hUC-MSCs combined fed-batch mode with medium refreshment to decrease concentrations of metabolic wastes, im

SUBMITTER: Dai Y 

PROVIDER: S-EPMC9207013 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature