Combination effects of capecitabine, irinotecan and 17-AAG on colorectal cancer cell line (HT-29).

ABSTRACT:

Objevtive

Evasion of apoptosis is a major feature of cancer cells, therefore designing treatment strategies to target apoptotic pathways seems effective. In this study, we investigate the effect of 17-AAG (17-allylaminogeldanamycin) alone and in double and triple combination with capecitabine (Cap) and irinotecan (IR) on HT-29 colon cancer cell line apoptosis.

Methods

Capase-3, 8, 9, p53 and NF-κB genes expression were analyzed by Real-time PCR. DNA laddering assay was performed to confirm Real-time PCR results.

Results

Our results showed that all single treatment groups elevated expression of caspase-3, 8, and 9 significantly and IR/Cap was the only double combination group that could upregulate caspase-8 and -9. NF-κB was down-regulated in single treatment and IR/Ca

SUBMITTER: Zeynali-Moghaddam S 

PROVIDER: S-EPMC9207062 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature