Photoacoustic imaging reveals mechanisms of rapid-acting insulin formulations dynamics at the injection site.

ABSTRACT:

Objective

Ultra-rapid insulin formulations control postprandial hyperglycemia; however, inadequate understanding of injection site absorption mechanisms is limiting further advancement. We used photoacoustic imaging to investigate the injection site dynamics of dye-labeled insulin lispro in the Humalog® and Lyumjev® formulations using the murine ear cutaneous model and correlated it with results from unlabeled insulin lispro in pig subcutaneous injection model.

Methods

We employed dual-wavelength optical-resolution photoacoustic microscopy to study the absorption and diffusion of the near-infrared dye-labeled insulin lispro in the Humalog and Lyumjev formulations in mouse ears. We mathematically modeled the experimental data to calculate the absorption rate constants and dif

SUBMITTER: Khadria A 

PROVIDER: S-EPMC9207296 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature