Characterizing region-specific glucose metabolic profile of the rodent brain using Seahorse XFe96 analyzer.

ABSTRACT: The brain is highly complex with diverse structural characteristics in accordance with specific functions. Accordingly, differences in regional function, cellular compositions, and active metabolic pathways may link to differences in glucose metabolism at different brain regions. In the current study, we optimized an acute biopsy punching method and characterized region-specific glucose metabolism of rat and mouse brain by a Seahorse XFe96 analyzer. We demonstrated that 0.5 mm diameter tissue punches from 180-µm thick brain sections allow metabolic measurements of anatomically defined brain structures using Seahorse XFe96 analyzer. Our result indicated that the cerebellum displays a more quiescent phenotype of glucose metabolism than cerebral cortex, basal ganglia, and hippocampus. In addi

SUBMITTER: Wang L 

PROVIDER: S-EPMC9207488 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature