MRNA and tRNA modification states influence ribosome speed and frame maintenance during poly(lysine) peptide synthesis.

ABSTRACT: Ribosome speed is dictated by multiple factors including substrate availability, cellular conditions, and product (peptide) formation. Translation slows during the synthesis of cationic peptide sequences, potentially influencing the expression of thousands of proteins. Available evidence suggests that ionic interactions between positively charged nascent peptides and the negatively charged ribosome exit tunnel impede translation. However, this hypothesis was difficult to test directly because of inability to decouple the contributions of amino acid charge from mRNA sequence and tRNA identity/abundance in cells. Furthermore, it is unclear if other components of the translation system central to ribosome function (e.g., RNA modification) influence the speed and accuracy of positively charged

SUBMITTER: Smith TJ 

PROVIDER: S-EPMC9207662 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature