Multiplexed quantification of insulin and C-peptide by LC-MS/MS without the use of antibodies.

ABSTRACT:

Introduction

The measurement of insulin and C-peptide provides a valuable tool for the clinical evaluation of hypoglycemia. In research, these biomarkers are used together to better understand hyperinsulinemia, hepatic insulin clearance, and beta cell function. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an attractive approach for the analysis of insulin and C-peptide because the platform is specific, can avoid certain limitations of immunoassays, and can be multiplexed. Previously described LC-MS/MS methods for the simultaneous quantification of insulin and C-peptide measure the intact analytes and most have relied on immunoaffinity enrichment. These approaches can be limited in terms of sensitivity and interference from auto-antibodies, respectively. We have deve

SUBMITTER: Foulon N 

PROVIDER: S-EPMC9207678 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature