Characterization of the Key Determinants of Phd Antitoxin Mediated Doc Toxin Inactivation in <i>Salmonella</i>.

ABSTRACT: In the search for novel antimicrobial therapeutics, toxin-antitoxin (TA) modules are promising yet underexplored targets for overcoming antibiotic failure. The bacterial toxin Doc has been associated with the persistence of Salmonella in macrophages, enabling its survival upon antibiotic exposure. After developing a novel method to produce the recombinant toxin, we have used antitoxin-mimicking peptides to thoroughly investigate the mechanism by which its cognate antitoxin Phd neutralizes the activity of Doc. We reveal insights into the molecular detail of the Phd-Doc relationship and discriminate antitoxin residues that stabilize the TA complex from those essential for inhibiting the activity of the toxin. Coexpression of Doc and antitoxin peptides in Salmonella was able to

SUBMITTER: de Castro GV 

PROVIDER: S-EPMC9207808 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature