Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free Zn².

ABSTRACT: Fluorescent Zn²⁺ sensors play a pivotal role in zinc biology, but their application in complex media such as blood serum or plate reader-based cellular assays is hampered by autofluorescence and light scattering. Bioluminescent sensor proteins provide an attractive alternative to fluorescent sensors for these applications, but the only bioluminescent sensor protein developed so far, BLZinCh, has a limited sensor response and a suboptimal Zn²⁺ affinity. In this work, we expanded the toolbox of bioluminescent Zn²⁺ sensors by developing two new sensor families that show a large change in the emission ratio and cover a range of physiologically relevant Zn²⁺ affinities. The LuZi platform relies on competitive complementation of split NanoLuc luciferas

SUBMITTER: Michielsen CMS 

PROVIDER: S-EPMC9207811 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature