A multilayer network model of neuron-astrocyte populations in vitro reveals mGluR₅ inhibition is protective following traumatic injury.

ABSTRACT: Astrocytes communicate bidirectionally with neurons, enhancing synaptic plasticity and promoting the synchronization of neuronal microcircuits. Despite recent advances in understanding neuron-astrocyte signaling, little is known about astrocytic modulation of neuronal activity at the population level, particularly in disease or following injury. We used high-speed calcium imaging of mixed cortical cultures in vitro to determine how population activity changes after disruption of glutamatergic signaling and mechanical injury. We constructed a multilayer network model of neuron-astrocyte connectivity, which captured distinct topology and response behavior from single-cell-type networks. mGluR₅ inhibition decreased neuronal activity, but did not on its own disrupt functional connec

SUBMITTER: Schroeder ME 

PROVIDER: S-EPMC9208011 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature